Human periodontal ligament stem cells with distinct osteogenic potential induce bone formation in rat calvaria defects-Reference-Cited by-同舟云学术

Human periodontal ligament stem cells with distinct osteogenic potential induce bone formation in rat calvaria defects

Published:2022-06 Issue:6 Volume:17 Page:341-353
ISSN:1746-0751
Container-title:Regenerative Medicine
language:en
Short-container-title:Regenerative Medicine

Author:

Adolpho Leticia Faustino¹^ORCID,Lopes Helena Bacha¹^ORCID,Freitas Gileade Pereira¹^ORCID,Weffort Denise¹^ORCID,Campos Totoli Gabriela Guaraldo¹^ORCID,Loyola Barbosa Ana Carolina¹^ORCID,Freire Assis Rahyza Inacio²^ORCID,Silverio Ruiz Karina Gonzales²^ORCID,Andia Denise Carleto³^ORCID,Rosa Adalberto Luiz¹^ORCID,Beloti Marcio Mateus¹^ORCID

Affiliation:

1. Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av do Café, s/n, Ribeirão Preto, SP, 14040-904, Brazil

2. Department of Prosthodontics & Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Av Limeira, 901, Piracicaba, SP, 13414-903, Brazil

3. Health Science Institute, Dental Research Division, Paulista University, Dr Bacelar St, 1212, São Paulo, SP, 04026-002, Brazil

Abstract

Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition to mixed cells, to repair bone tissue. Methods: Cell phenotype, proliferation and differentiation were evaluated. Undifferentiated PDLSCs were injected into rat calvarial defects and the new bone was evaluated by μCT, histology and real-time PCR. Results: PDLSCs exhibited a typical mesenchymal stem cell phenotype and HP-PDLSCs showed lower proliferative and higher osteogenic potential than LP-PDLSCs. PDLSCs induced similar bone formation and histological analysis suggests a remodeling process, confirmed by osteogenic and osteoclastogenic markers, especially in tissues derived from defects treated with HP-PDLSCs. Conclusion: PDLSCs induced similar bone formation irrespective of their in vitro osteogenic potential.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Amparo à Pesquisa do Estado de São Paulo

Publisher

Future Medicine Ltd

Subject

Embryology,Biomedical Engineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/rme-2021-0178

Reference42 articles.

1. Autograft, Allograft, and Bone Graft Substitutes: Clinical Evidence and Indications for Use in the Setting of Orthopaedic Trauma Surgery

2. Advances in osteobiologic materials for bone substitutes