Confocal reflectance microscopy for metal and lipid nanoparticle visualization in the brain

Author:

Cakir-Aktas Canan1ORCID,Erdener Sefik Evren1ORCID,Teke Büşra2ORCID,Bozdag Pehlivan Sibel2ORCID,Zeybek Naciye Dilara3ORCID,Taskiran-Sag Aslihan1ORCID,Kaya Zeynep1ORCID,Dalkara Turgay1ORCID,Mut Melike14ORCID

Affiliation:

1. Hacettepe University, Institute of Neurological Sciences & Psychiatry, Ankara, 06230, Turkey

2. Department of Pharmaceutical Technology, Hacettepe University, Faculty of Pharmacy, Ankara, 06230, Turkey

3. Department of Histology & Embryology, Hacettepe University, Faculty of Medicine, Ankara, 06230, Turkey

4. Department of Neurosurgery, Hacettepe University, Faculty of Medicine, Ankara, 06230, Turkey

Abstract

Aim: A requirement for nanoparticle (NP) research is visualization of particles within cells and tissues. Limitations of electron microscopy and low yields of NP fluorescent tagging warrant the identification of alternative imaging techniques. Method: Confocal reflectance microscopy (CRM) in combination with fluorescence imaging was assessed for visualizing rhodamine B-conjugated silver and fluorescein isothiocyanate-conjugated lipid core-stearylamine NP uptake in vitro and in vivo. Results: CRM successfully identified cellular uptake and blood–brain barrier penetration of NPs owing to their distinguishing refractive indices. NP-dependent reflectance signals in vitro were dose and incubation time dependent. Finally, CRM facilitated the distinction between nonspecific fluorescence signals and NPs. Conclusion: These findings demonstrate the value of CRM for NP visualization in tissues, which can be performed with a standard confocal microscope.

Funder

Bilim Akademisi

Türkiye Bilimler Akademisi

Hacettepe Üniversitesi

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Optics and Actuation Co-Design for an Implantable Reflectance Confocal Microscope with Large Working Distance;2022 IEEE/ASME International Conference on Advanced Intelligent Mechatronics (AIM);2022-07-11

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