How fluorescent labelling alters the solution behaviour of proteins
Author:
Affiliation:
1. Department of Chemistry
2. Maynooth University
3. Maynooth
4. Ireland
5. CNR-ISC UOS Sapienza
6. 00186 Roma
7. Italy
8. Dipartimento di Fisica
9. “Sapienza” Universita’ di Roma
10. Roma
Abstract
The impact of adding a fluorescent dye to a protein is examined using protein phase diagrams and numerical simulations. The addition of the dye is the equivalent of adding a large attractive patch to the surface of the protein, which results in significant changes to the protein solution behaviour.
Funder
Science Foundation Ireland
Irish Research Council for Science, Engineering and Technology
Publisher
Royal Society of Chemistry (RSC)
Subject
Physical and Theoretical Chemistry,General Physics and Astronomy
Link
http://pubs.rsc.org/en/content/articlepdf/2015/CP/C5CP04463D
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