Effect of fluoxetine on planktonic and biofilm growth and the antimicrobial susceptibility of <i>Burkholderia pseudomallei</i>-Reference-Cited by-同舟云学术

Effect of fluoxetine on planktonic and biofilm growth and the antimicrobial susceptibility of Burkholderia pseudomallei

Published:2023-08 Issue:12 Volume:18 Page:785-794
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Guedes Gláucia MM¹²^ORCID,Araújo Emanuela S²^ORCID,Ribeiro Késia VC²^ORCID,Pereira Vinícius C²^ORCID,Soares Ana CCF²,Freitas Alyne S²^ORCID,Amando Bruno R²^ORCID,Cordeiro Rossana A¹^ORCID,Rocha Marcos FG¹³^ORCID,Sidrim José JC¹^ORCID,Castelo-Branco Débora SCM¹²^ORCID

Affiliation:

1. Department of Pathology & Legal Medicine, Postgraduate Program in Medical Microbiology, Laboratory of Emerging & Reemerging Pathogens, Federal University of Ceara, Rua Cel, Nunes de Melo, 1315 – Rodolfo Teófilo – CEP 60430–275, Fortaleza, Ceará, Brazil

2. Department of Pathology & Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceara, Rua Cel, Nunes de Melo, 1315 – Rodolfo Teófilo – CEP 60430–275, Fortaleza, Ceará, Brazil

3. College of Veterinary, State University of Ceara. Av. Dr Silas Munguba, 1700, Campus do Itaperi – CEP 60714–903, Fortaleza, Ceará, Brazil

Abstract

Aim: This study evaluated the effect of fluoxetine (FLU) on planktonic and biofilm growth and the antimicrobial susceptibility of Burkholderia pseudomallei. Materials & methods: The minimum inhibitory concentrations (MICs) for FLU were determined by broth microdilution. Its effect on growing and mature biofilms and its interaction with antibacterial drugs were evaluated by assessing biofilm metabolic activity, biomass and structure through confocal microscopy. Results: The FLU MIC range was 19.53–312.5 μg/ml. FLU eradicated growing and mature biofilms of B. pseudomallei at 19.53–312.5 μg/ml and 1250–2500 μg/ml, respectively, with no structural alterations and enhanced the antibiofilm activity of antimicrobial drugs. Conclusion: These results bring perspectives for the use of FLU in the treatment of melioidosis, requiring further studies to evaluate its applicability.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2022-0272

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