ANALYSIS OF SKIN SCARRING WITH PRE-, INTRA- AND DELAYED SURGICAL INJECTION THERAPY: THE PRECLINICAL RESEARCH

Abstract

Purpose: Experimental testing of injection therapy in the area of the upcoming skin incision in order to improve the quality of achieving the aesthetic appearance of the skin scarring process. Materials and methods: The study was carried out on the skin of the sternum and anterior abdominal wall on 2 closely related minipigs.3 series of operations were carried out synchronously on two individuals in a surgical operating room with preliminary specific markings in the form of rectangles and trapezoids, respectively; the nipple-areolar complexes served as a guide for drawing the figures. At each stage, the same type of geometric marking of the surgical field was used and repeated interventions were performed in the same areas of injection therapy and biopsy sampling. In the postoperative period, on the 30th and 120th days, biopsy specimens were taken to verify the ongoing reparative processes based on clinical, photometric and histological analysis. Results: Preoperative injection therapy contributed to the formation of a normotrophic scar in structure closer to normal skin compared to a scar during normal tissue healing, which allows it to be recommended for preoperative preparation and for research in humans. The selected technique allows for assessment of the classic primary wound, with the introduction of saline and botulinum toxin intraoperatively and delayed at 1 and 4 months with histological analysis of the results. At each stage of the experiment, the team achieved long-term survival of minipigs without compromising their vital functions. The same type of geometric marking of the surgical field was used and repeated interventions were performed in the same areas of injection therapy and biopsy sampling. Conclusion: The methodology allows to assess different methods of linear scar formation with or without the use of injection therapy during surgical access, as well as comparing tissue adhesion histologically and optimal scar formation. When the effectiveness of BTA therapy is achieved, injection therapy requires further research under conditions of tissue deficit or tension during wound closure.