Affiliation:
1. Sevastopol State University, Sevastopol, Russia
2. Kovalevsky Institute of Marine Biological Research RAS – IMBR, Sevastopol, Russia
Abstract
Mathematical decomposition of the absorption spectrum of the culture of the red microalga Porphyridium purpureum (Bory) Ross was carried out in the spectral range from 550 to 750 nm. The culture spectra were recorded on a spectrophotometer with an integrating sphere. Using the literature data, mathematical models of the absorption spectra of the following pigments were compiled: B-phycoerythrin, R-phycocyanin, allophycocyanin and chlorophyll a. The mathematical model of each pigment represented the sum of Gaussian curves. The resulting equations were taken as a basis for modeling the native spectrum. A general model of the absorption spectrum of culture in the region of 550–750 nm has been compiled, consisting of the sum of individual pigments. Using the model obtained in practice, it is possible to determine the concentrations of chlorophyll a, and individual phycobilin pigments directly from the absorption spectrum of the culture.
Reference28 articles.
1. Arnon D.I., McSwain B.D., Tsujimoto H.Y., Wada K. Photochemical activity and components of membrane preparations from blue-green algae. I. Coexistence of two photosystems in relation to chlorophyll a and removal of phycocyanin. Bioch. Biophys. Acta. 1974. V.357, №2. P. 231–245. DOI: 10.1016/0005-2728(74)90063-2
2. Barsanti L., Evangelista V., Frassanito A. M., Vesentini N., Passarelli V., Gualtieri P. Absorption microspectroscopy, theory and applications in the case of the photosynthetic compartment. Micron. 2007. Т.38, №3. P. 197–213.
3. Chernyshev D.N., Klochkova V.S. Razdelenie nativnogo spektra pogloshcheniya kul'tury mikrovodorosli Spirulina platensis [Separation of the native absorption spectrum of the microalgae culture Spirulina platensis]. Aktual'nye voprosy biologicheskoj fiziki i himii. 2021. V.6, №2. P. 217–222. (In Russ.)
4. Dyhrman S.T. Nutrients and their acquisition: phosphorus physiology in microalgae. Dev. Appl. Phycol. 2016. V.6. DOI: 10.1007/978–3–319–24945–2_8
5. Flynn K.J. A mechanistic model for describing dynamic multi-nutrient, light, temperature interaction in phytoplankton. J. Plan. Res. 2001. V.23. P. 977–997.
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