Optimization of approaches in the development of microbiological feed for mariculture

Author:

Matrosova Inga1,Panchishina E.2,Politaeva Anastasiya1,Kornienko Nadezhda Leonidovna3

Affiliation:

1. Federal State Budget Educational Institution of Higher Education Far Eastern State Technical Fisheries University

2. FSBEU «The Far Eastern State Technical Fisheries University»

3. Far Eastern State Technical Fisheries University

Abstract

The paper presents the results of selecting methods for establishing the growth rate, generation time and doubling of the biomass of yeast cells Rhodotorula spp., which are part of probiotic feed preparations for aquaculture objects. The identified atypical period of the lag phase was observed on both studied nutrient media (Lundin, YEPD), where instead of an increase in the biomass of the culture, some of the introduced cells died. The results obtained provide the basis for further study and search for new highly productive strains of Rhodotorula spp., selection of technological regimes for obtaining feed preparations based on them, modification and optimization of the composition of the culture medium to increase the growth of yeast biomass.

Publisher

FSBI TSUREN

Subject

General Medicine

Reference11 articles.

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2. Lone Gram, J. Melchiorsen, Bettina Spanggaard, I. Huber, T.F. Nielsen. Inhibition of Vibrio anguillarum by Pseudomonas flourescens AH2, a possible probiotic treatment of fish // Applied and Environmental Microbiology. 1999. Vol. 65. Р. 969-973., Lone Graham, J. Melchiorsen, Bettina Spanggaard, I. Huber, T.F. Nielsen. (1999). Inhibition of Vibrio anguillarum Pseudomonas flourescens AH2 - a possible way to treat fish with probiotics // Applied Microbiology and ecological Microbiology. Volume 65. Pp. 969-973. (In Russ.).

3. Ji-hui Wang, Liu-qun Zhao, Jin-feng Liu, Han Wang, Shan Xiao. Effect of potential probiotic Rhodotorula benthica D30 on the growth performance, digestive enzyme activity and immunity in juvenile sea cucumber Apostichopus japonicus // Fish Shellfish Immunol. 2015. Vol. 43, № 2. P. 330-336. DOI 10.1016/j.fsi.2014.12.028., Ji-hui Wang, Liu-qun Zhao, Jin-feng Liu, Han Wang, Shan Xiao. 2015. The effect of the potential probiotic Rhodotorula benthica D30 on growth indicators, activity of digestive enzymes and immunity of juvenile sea cucumber Apostichopus japonicus // Immunol of fish and shellfish. Volume 43, No. 2. Pp. 330-336. doi: 10.1016/j.fsi.2014.12.028.

4. Политаева А.А. Применение красных дрожжей R. benthica при культивировании личинок дальневосточного трепанга // Комплексные исследования в рыбохозяйственной отрасли: материалы V Международной научно-технической конференции студентов, аспирантов и молодых ученых. Владивосток. 2020. С. 33-35., Politaeva A.A. (2020). The use of red yeast R. benthica in the cultivation of larvae of the Far Eastern sea cucumber // Complex research in the fisheries industry: materials in the scientific and technical International Conference of students, postgraduates and young scientists. Vladivostok. Pp. 33-35. (In Russ.).

5. Matrosova I.V., Panchishina E.M., Politaeva A.A., Kornienko N.L. Use the Rhodotorula benthica yest to feeding of echinoderms // IOP Conference Series: Earth and Enviromental Science, Krasnoyarsk, 18-20 ноября 2020 года / Krasnoyarsk Science and Technology City Hall. Krasnoyarsk: IOP Publishing Ltd. № 677. 2021.P. 42029. DOI 10.1088/1755-1315/677/4/042029., Matrosova I.V., Panchishina E.M., Politaeva A.A., Kornienko N.L. (2021). Use Rhodotorula benthica yest for feeding echinoderms // IOP Conference Series: Earth and Environment Science, Krasnoyarsk, November 18-20, 2020 / Krasnoyarsk Scientific and Technical City Hall. Krasnoyarsk: VGD Publishing House LLC. No. 677. p. 42029. DOI 10.1088/1755-1315/677/4/042029.

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