Relationship of leptin concentrations as part of cytokine response to hyperlipoproteidemia in practically healthy northerners

Abstract

BACKGROUND: Cytokines secrete all cells when there is a threat of damage to it. Leptin, having all the properties of a cytokine, produces fat cells. It was of interest to identify the ratio of leptin concentrations and cytokines activating preventive inflammatory reactions in the blood of practically healthy Northerners with hyperlipoproteidemia.AIM: To identify the ratio of concentrations of leptin and IL-1ß, TNF-α, IL-6 and IL-10 in the blood of practically healthy Northerners with hyperlipoproteidemia.MATERIAL AND METHODS: A survey of 286 practically healthy people aged 35-55 years living in the Arkhangelsk region was conducted. The body mass index in persons of the first group (n=211) was in the range of 19.5-26.8 kg/m2 and corresponded to the norm; and 75 people of the second group (body mass index 31-40 kg/m2). Concentrations of leptin, interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) were determined in the blood by ­enzyme immunoassay on an automatic Evolis enzyme immunoassay analyzer from Bio-Rad (Germanytotal ­cholesterol (OH), apolipoprotein A-1 (ApoA-1), apolipoprotein B (ApoV), high-density lipoproteins (HDL), low-density lipoproteins (LDL), very low-density lipoproteins (VLDL), oxidized low-density lipoproteins (LDL), triglycerides (TG), phospholipids (FL) on the biochemical analyzer «Stat fax 1904 Plus» of the company «Awareness Technology, Inc.» USA.RESULTS: Fasting hyperlipoproteidemia in practically healthy people is associated with an increase in the concentration of leptin in the blood within the physiological content. The closest relationship between an increase in leptin levels and concentrations of proinflammatory cytokines IL-1ß, TNF-α and IL-6 was established (r=0.68, 0.74 and 0.83, respectively; p<0.001). The severity of the reaction of proinflammatory cytokines is regulated by a parallel increase in the secretion of anti-inflammatory IL-10 (r=0.62; p<0.001).CONCLUSION: It should be recognized that the stimulation of leptin secretion is provided by the influence of more intense signals initiating a cytokine reaction. In order to ensure the secretion of leptin, compared with that of proinflammatory cytokines, the influence of more pronounced signals associated with an increased need for metabolic energy is required. Higher concentrations of leptin were found with a low plasma content of HDL, ApoA-1 ligands and ApoB; The opinion is substantiated that leptin in physiological concentrations regulates the use of the depot of the energy substrate of adipose tissue by increasing its secretion with a low content of HDL in plasma, ligands of lipid transport forms.