Affiliation:
1. Universidade Estadual de Campinas, Brasil
Abstract
The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.
Reference24 articles.
1. Lipases: their structure, biochemistry and application;Aires-Barros MR,1994
2. Partition of cell particles and macromolecules;Albertsson P. -Å.,1986
3. Como Fazer Experimentos: Pesquisa e Desenvolvimento na Ciência e na Indústria;Barros Neto B.,2001
4. Planejamento e otimização de experimentos;Barros Neto B,1995
5. Optimization of drying process of Zea mays malt to use as alternative source of amylolytics enzymes;Biazus J. P. M.;Brazilian Archive of Biology and Technology,2005
Cited by
22 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献