Paternity in Brazilian goats through the use of DNA microsatellites

Author:

Araújo Adriana Mello de1,Guimarães Simone Eliza Facioni2,Pereira Carmen Silva2,Lopes Paulo Sávio2,Rodrigues Marcelo Teixeira2,Machado Théa Mírian Medeiros2

Affiliation:

1. Universidade Federal de Viçosa; Embrapa Meio-Norte

2. Universidade Federal de Viçosa

Abstract

A total of 292 animals from three breeds (Alpine and Saanen dairy breeds, and the Brazilian naturalized breed Moxotó) were genotyped, comprising 276 paternity cases. Statistical analyses were carried out by using TFPGA and CERVUS programs. Heterozygosis ranged from 0.542 (ILSTS005) to 0.825 (INRA006), with an average of 0.717 for all loci. Polymorphic information content (PIC) was 0.676 and 0.542, and combined exclusion probabilities (EP) were 0.999591 and 0.988375 for known and unknown maternal genotypes, respectively. The microsatellite system reveals 10% of paternity misidentification in evaluated registers.

Publisher

FapUNIFESP (SciELO)

Subject

Animal Science and Zoology

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5. Performing the exact test of Hardy-Weinberg proportion for multiple alleles;GUO S.W.;Biometrics,1992

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