Verification of Pedigree Information with Paternity Testing in Karacabey Merino Sheep

Abstract

Abstract In recent years, complex tests of DNA sequences, genetic research and the use of specific DNA polymorphisms as genetic markers have been developing rapidly. It is widely used in DNA-based parental testing. Therefore, it has replaced the blood types routinely used in pedigree tests. The aim of the study was to evaluate the 14 microsatellite loci in the study in terms of paternity test parameters and to investigate the usability of the relevant loci in determining the parentage of the Karacabey Merino breed. In the study, paternity testing was performed on 5 rams of Karacabey Merino breed and their probable 98 offspring using 14 microsatellite loci. 290 alleles were observed in the study. The observed heterozygosity ratio (Ho) on the basis of loci was formed between 0.82 and 0.98, and the expected heterozygosity ratio (She) was between 0.87 and 0.93. In the study, the individual exclusion probabilities (PE) value of the loci were between 0.596 and 0.761 and the combined exclusion probabilities (CPE) values of 0.99999995 were calculated. According to the results of the study, it has been revealed that paternity tests using 14 microsatellite markers can be used with high accuracy.