Resistance of transgenic papaya trees to papaya ringspot in Brazil

Author:

Meissner Filho Paulo Ernesto1ORCID,Vilarinhos Alberto Duarte1ORCID,Oliveira Vania Jesus dos Santos de2ORCID,Silva Dreid de Cerqueira Silveira da3ORCID,Santos Vanderlei da Silva1ORCID,Dantas Jorge Luiz Loyola2ORCID

Affiliation:

1. Embrapa Mandioca e Fruticultura, Brazil

2. Faculdade Maria Milza, Brazil

3. Universidade Federal do Recôncavo da Bahia, Brazil

Abstract

Abstract: The objective of this work was to evaluate the resistance of transgenic papaya populations (PTPs) to Papaya ringspot virus-P (PRSV-P). 'Sunrise Solo' transgenic papaya plants were produced with the gene of the PRSV-P protein coat, and PRSV was mechanically inoculated in plants in greenhouse conditions. The presence of the CP/PRSV gene and homozygosis were evaluated by polymerase chain reaction (PCR). Selected plants and the 'Sunrise Solo' control were transplanted to the field for agronomic evaluations. The plants evaluated in greenhouse conditions showed resistance between 96.3 and 5.8%, without variation of symptoms. The PTPs 1/6, 1/7, 1/9, 1/10, 1/15, 2/38, 2/41, 2/56, 2/65, 3/27, 3/46, 3/48, 4/9, 4/27, 8/4, 8/23, 8/33, 18/3, 18/4, 18/8, 18/22, 18/27, 28/97, 28/104, and 28/110 showed no symptoms, they were ELISA negative, and most of them contained the CP and NPT II genes. PTPs 1/6 and 3/46 had the CP gene in homozygosis and in double insertion. PTPs 1/6/20, 1/6/59, 1/6/64, 1/6/90, 3/46/44, 3/46/52, and 18/27/97 had a well-formed fruit cluster, piriform fruit weighing approximately 500 grams, orange pulp, and less than 10% carpelloidy. PTPs 1/6/59 and 3/46/52 show resistance to PRSV, good agronomic characteristics, and the CP gene in homozygosis.

Publisher

FapUNIFESP (SciELO)

Subject

Agronomy and Crop Science,Animal Science and Zoology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Tropical Fruit Virus Resistance in the Era of Next-Generation Plant Breeding;SynBio;2024-07-08

2. Papaya—II;Viral Diseases of Field and Horticultural Crops;2024

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