Affiliation:
1. 1 University of Kaposvár Department of Physiology, Faculty of Animal Science Kaposvár Hungary
Abstract
The effect of pre-treatment with Escherichia coli O83 lipopolysaccharide (LPS) on concanavalin A-induced ascites was examined. The LPS was injected intraperitoneally (i.p.) in different doses to mice, and then ascites was induced by i.p. administration of concanavalin A (ConA) (25 mg/kg b.w.). After 2.5 h the mice were killed and the ascitic fluid was collected and measured. The LPS produced a marked and dose-dependent inhibition of ConA-induced ascites and the effect of pre-treatment lasted up to almost a week. Complete inhibition could not be achieved. If administered alone, LPS did not produce ascites.It is well known that LPS enhances vascular permeability in several tissues, but the present work shows that peritoneal permeability is not enhanced by this agent. Suppression of ConA-induces ascites may be explained by the hypotonic effect of LPS.
Subject
General Immunology and Microbiology,General Medicine
Reference15 articles.
1. Inflammatory ascites formation induced by macromolecules in mice and rats;Baintner K.;Am J Physiol Regul Integr Comp Physiol,2009
2. Bacterial lipopolysaccharides: Extraction with phenol-water and further applications of the procedure;Westphal O.;Methods Carbohydr Chem,1965
3. Ultrahistochemical demonstration of saccharide components of complex carbohydrates at the alveolar cell surface and at the mesothelial cell surface of the pleura visceralis of mice by means of concanavalin A;Roth J.;Exp Pathol (Jena),1973
4. Effect of intraperitoneally administered plant lectins on leukocyte diapedesis and visceral organ weight in rats and mice;Baintner K.;Pathol Oncol Res,2007
5. Interaction of concanavalin A with bacterial lipopolysaccharides in agarose gel;Baintner K.;Acta Microbiol Immunol Hung,2011
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