Polymerase chain reaction amplification of 16S-23S spacer region for rapid identification of Salmonella serovars-Reference-Cited by-同舟云学术

Polymerase chain reaction amplification of 16S-23S spacer region for rapid identification of Salmonella serovars

Published:2002-05-01 Issue:2 Volume:50 Page:161-166
ISSN:0236-6290
Container-title:Acta Veterinaria Hungarica
language:
Short-container-title:

Author:

Bakshi C. S.¹,Singh V. P.²,Malik Meenakshi³,

Affiliation:

1. 1 National Biotechnology Centre, Indian Veterinary Research Institute Izatnagar-243 122, Bareilly, UP, India

2. 2 Division of Bacteriology and Mycology, Indian Veterinary Research Institute Izatnagar-243 122, Bareilly, UP, India

3. 3 National Biotechnology Centre, Indian Veterinary Research Institute Izatnagar-243 122, Bareilly, UP, India

Abstract

Polymerase chain reaction (PCR) was used to amplify the spacer regions between the 16S and 23S genes of rRNA genetic loci of Salmonella serovars for their rapid identification. These genetic loci revealed a significant level of polymorphism in length across the species/serovar lines. When the 16S-23S spacer region amplification products were subjected to agarose electrophoresis, the patterns observed could be used to distinguish all the serovars of Salmonella tested. Unique elements obtained in amplification products were mostly clustered at serovar level, although certain genus-specific patterns were also observed. On the basis of the results obtained, the amplification of 16S-23S ribosomal spacer region could suitably be used in a PCR-based identification method for Salmonella serovars.

Publisher

Akademiai Kiado Zrt.

Subject

General Veterinary

Link

https://www.akademiai.com/doi/pdf/10.1556/avet.50.2002.2.5

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