Development of a highly sensitive HPLC method for the simultaneous determination of eight biogenic amines in aquatic products

Abstract

AbstractA highly sensitive high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of eight biogenic amines in aquatic products. The biogenic amines in the sample were extracted with 5% trichloroacetic acid, derived with dansyl chloride (Dns-Cl) and quantified by a UV detector. The results showed that tryptamine (TRY), 2-phenethylamine (PHE), putrescine (PUT), cadaverine (CAD), histamine (HIS), tyramine (TPY), spermidine (SPD), and spermine (SPM) were effectively separated in 18 min in the range of 0.1–50 mg/kg with a good linear coefficient (r2 > 0.999). The detection limits (LODs) of the eight biogenic amines were 0.007–0.021 mg/kg while the limits of quantification (LOQs) were 0.024–0.069 mg/kg with the recoveries basically between 68 and 123%. The determination of eight biogenic amines in five commercial fermented aquatic products indicating that the developed method could be applied for the simultaneous detection of biogenic amines in multiple aquatic products.