Evaluation of a Soybean Lectin–based Method for the Enrichment of Erythroblasts-Reference-Cited by-同舟云学术

Evaluation of a Soybean Lectin–based Method for the Enrichment of Erythroblasts

Published:2005-03 Issue:3 Volume:53 Page:329-330
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Babochkina Tatiana¹,Mergenthaler Susanne¹,Lapaire Olaf²,Kiefer Vivian¹,Yura Hirofumi³,Koike Kimie³,Holzgreve Wolfgang¹²,Hahn Sinuhe¹

Affiliation:

1. Laboratory for Prenatal Medicine, University Women's Hospital/Department of Research, University of Basel, Basel, Switzerland

2. University Women's Hospital, University of Basel, Basel, Switzerland

3. NeTech, Inc., Kanagawa, Japan

Abstract

We performed a comparative study of the enrichment of erythroblasts by a soybean agglutinin galactose-specific lectin method and a standardized magnetic cell-sorting (MACS) protocol. Blood samples, obtained from 11 pregnant women at between 11 and 40 weeks of gestation, were split and examined by each method in parallel. The number of erythroblasts recovered by the lectin method was approximately eightfold higher than the number obtained by MACS. Our data suggest that the lectin-based method may provide a better approach for the enrichment of rare fetal erythroblasts from maternal blood.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1369/jhc.4B6408.2005

Reference6 articles.

1. Fetal gender and aneuploidy detection using fetal cells in maternal blood: analysis of NIFTY I data

2. Prenatal Diagnosis Using Fetal Cells and Cell-Free Fetal DNA in Maternal Blood: What Is Currently Feasible?

3. [26] Single cell PCR in laser capture microscopy

4. New technique using galactose-specific lectin for isolation of fetal cells from maternal blood

5. Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Cited by 20 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Fetal nucleated red blood cells in maternal peripheral blood for non-invasive prenatal diagnosis;Chinese Science Bulletin;2023-07-12

2. Noninvasive prenatal diagnosis targeting fetal nucleated red blood cells;Journal of Nanobiotechnology;2022-12-30

3. Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron‐Transfer, Carbohydrate‐Mimetic/Cross‐Linker‐Peptide‐Collagen Film;Electroanalysis;2021-11-17

4. Direct Assessment of Single-Cell DNA Using Crudely Purified Live Cells: A Proof of Concept for Noninvasive Prenatal Definitive Diagnosis;The Journal of Molecular Diagnostics;2020-02

5. Concentration and extraction chip of fetal nucleated red blood Cells (NRBCs) by micro gap with diaphragm for fetal DNA diagnosis from maternal blood;Microsystem Technologies;2017-02-10