Selective Attachment of Synthetic DNA to Self-Assembled-Monolayer Functionalized Surfaces

Abstract

ABSTRACTSelf-assembled monolayer (SAM) films of organosilanes have been characterized as surfaces for the spatially-controlled, selective attachment of single-stranded synthetic DNA. Geometric patterns of DNA on silica substrates have been created by photochemical modification of the SAMs to produce regions which promote or resist DNA attachment. The attachment of DNA to the modified surfaces was followed using UV spectroscopy, radioassay, ellipsometry, and enzyme-linked colorimetric assay. We have also demonstrated that immobilized DNA is capable of hybridization with a complementary strand under stringent conditions.