Author:
Hu Zhi,Song Hao,Feng Jie,Zhou Cong,Yang Mei-Jie,Shi Pu,Yu Zheng-Lin,Li Yong-Ren,Guo Yong-Jun,Li Hai-Zhou,Zhang Tao
Abstract
Heat shock protein 70 (HSP70) members participate in a wide range of housekeeping and stress-related activities in eukaryotic cells. In marine ecosystems, bivalves encounter abiotic stresses, including high temperatures and low dissolved oxygen. Here, 133 MmHSP70 genes were identified through combined methods including Blastp, HMM and manual filtration, based on the whole Mercenaria mercenaria genome. The MmHSP70 genes were unevenly distributed, and 41 genes (33.08%) were located on Chr 7. Phylogenetic analyses indicated that the MmHSP70 gene family mainly consisted of two clusters and the Hspa12 subfamily underwent lineage-specific expansion. A high-density collinear gene block was observed between M. mercenaria Chr 7 and Cyclina sinensis Chr 14. Tandem duplication MmHSP70 gene pairs experienced different levels of purifying selection, which could be an important source of sequence and functional constraints. MmHSP70 genes showed tissue-specific and stress-specific expression. Most tandem duplication HSP70 gene pairs had high expression under hypoxia stress. HSP70 B2 tandem duplication gene pairs showed significantly increased expression under heat plus severe hypoxia stress. This study provided a comprehensive understanding of the MmHSP70 gene family in the M. mercenaria and laid a significant foundation for further studies on the functional characteristics of MmHSP70 genes during exposure to heat and hypoxia stress.
Funder
National Key Research and Development Program of China
Tianjin Science and Technology Program
Tianjin Agriculture and Rural Committee
Agriculture Research System of China
Shandong Province Key Agricultural Project for Application Technology Innovation
Taishan Industry Leading Talents
Yantai Science and Technology Bureau
Polit National Laboratory for Marine Science and Technology
Subject
Ocean Engineering,Water Science and Technology,Aquatic Science,Global and Planetary Change,Oceanography
Cited by
16 articles.
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