Author:
Yao Xue,Liao Baixue,Chen Feng,Liu Lüye,Wu Kaiwen,Hao Yaying,Li Yanping,Wang Yuebin,Fan Ruiling,Yin Jun,Liu Lei,Guo Yuanbiao
Abstract
Graphical AbstractIn our study, three methods were used for isolating extracellular vesicles derived from pleural effusion (pEVs), including ultracentrifugation (UC), a combination of UC and size exclusion chromatography (UC-SEC) and a combination of UC and density gradient ultracentrifugation (UC-DGU), and followed with proteomics analysis. Our results show that the method of UC-SEC could significantly improve the purity of pEVs and improve the performance of proteomic analysis of pEVs. The exosomal proteins CD11C, HLA DPA1 and HLA DRB1 may act as potential markers of pEVs.
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology