Author:
Lyu Zhongfan,Hao Yongchao,Chen Liyang,Xu Shoushen,Wang Hongjin,Li Mengyao,Ge Wenyang,Hou Bingqian,Cheng Xinxin,Li Xuefeng,Che Naixiu,Zhen Tianyue,Sun Silong,Bao Yinguang,Yang Zujun,Jia Jizeng,Kong Lingrang,Wang Hongwei
Abstract
Even frequently used in wheat breeding, we still have an insufficient understanding of the biology of the products via distant hybridization. In this study, a transcriptomic analysis was performed for six Triticum aestivum-Thinopyrum elongatum substitution lines in comparison with the host plants. All the six disomic substitution lines showed much stronger “transcriptomic-shock” occurred on alien genomes with 57.43–69.22% genes changed expression level but less on the recipient genome (2.19–8.97%). Genome-wide suppression of alien genes along chromosomes was observed with a high proportion of downregulated genes (39.69–48.21%). Oppositely, the wheat recipient showed genome-wide compensation with more upregulated genes, occurring on all chromosomes but not limited to the homeologous groups. Moreover, strong co-upregulation of the orthologs between wheat and Thinopyrum sub-genomes was enriched in photosynthesis with predicted chloroplastic localization, which indicates that the compensation happened not only on wheat host genomes but also on alien genomes.
Funder
National Natural Science Foundation of China
Agricultural Variety Improvement Project of Shandong Province