Author:
Corbière Véronique,Lambert Eleonora E.,Rodesch Marine,van Gaans-van den Brink Jacqueline A. M.,Misiak Alicja,Simonetti Elles,Van Praet Anne,Godefroid Audrey,Diavatopoulos Dimitri A.,van Els Cécile A. C. M.,Mascart Françoise,
Abstract
IntroductionThe characterization of B. pertussis (Bp) antigen-specific CD4+ T cell cytokine responses should be included in the evaluation of immunogenicity of pertussis vaccines but is often hindered by the lack of standardized robust assays.MethodsTo overcome this limitation, we developed a two-step assay comprising a short-term stimulation of fresh whole blood with Bp antigens and cryopreservation of the stimulated cells, followed later on by batch-wise intracellular cytokine analysis by flow cytometry. Blood samples collected from recently acellular (aP) vaccine boosted subjects with a whole-cell- or aP-primed background was incubated for 24 hrs with Pertussis toxin, Filamentous hemagglutinin or a Bp lysate (400µl per stimulation). Antigen-specific IFN-γ-, IL-4/IL-5/IL-13-, IL-17A/IL-17F- and/or IL-22-producing CD4+ T cells were quantified by flow cytometry to reveal Th1, Th2, and Th17-type responses, respectively. The frequencies of IFN-γ-producing CD8+ T cells were also analyzed.ResultsWe demonstrate high reproducibility of the Bp-specific whole blood intracellular staining assay. The results obtained after cryopreservation of the stimulated and fixed cells were very well correlated to those obtained without cryopreservation, an approach used in our previously published assay. Optimization resulted in high sensitivity thanks to very low non-specific backgrounds, with reliable detection of Bp antigen-specific Th1, Th2 and Th17-type CD4+ T cells, in the lowest range frequency of 0.01-0.03%. Bp antigen-specific IFN-γ+ CD8+ T lymphocytes were also detected. This test is easy to perform, analyse and interpret with the establishment of strict criteria defining Bp antigen responses.DiscussionThus, this assay appears as a promising test for evaluation of Bp antigen-specific CD4+ T cells induced by current and next generation pertussis vaccines.
Subject
Immunology,Immunology and Allergy
Reference56 articles.
1. Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to bordetella pertussis and other bordetella subspecies;Mattoo;Clin Microbiol Rev,2005
2. Chapter 16: Pertussis. In: Centers for disease control and prevention;Havers,2021
3. A controlled trial of a two-component acellular, a five-component acellular, and a whole-cell pertussis vaccine;Gustafsson;N Engl J Med,1996
4. Association of childhood pertussis with receipt of 5 doses of pertussis vaccine by time since last vaccine dose, California, 2010;Misegades;JAMA,2012
5. Unexpectedly limited durability of immunity following acellular pertussis vaccination in preadolescents in a north American outbreak;Witt;Clin Infect Dis,2012
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