In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells

Author:

Gao Shuo,Zhao Lei,Fan Zhiqiang,Kodibagkar Vikram D.,Liu Li,Wang Hanqin,Xu Hong,Tu Mingli,Hu Bifu,Cao Chuanbin,Zhang Zhenjian,Yu Jian-Xin

Abstract

For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping.

Funder

National Natural Science Foundation of China

Publisher

Frontiers Media SA

Subject

General Chemistry

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