Author:
Yuan Yangyang,Liu Shaohua,Ding Xianfei,Li Ying,Zhang Xiaojuan,Song Heng,Qi Xueyan,Zhang Zihao,Guo Kaiyuan,Sun Tongwen
Abstract
BackgroundThe mortality rate associated with sepsis in elderly individuals is higher than that in younger individuals. The intestinal microbiota has been demonstrated to play an important role in the occurrence and development of sepsis. The purpose of this study was to investigate the differences in the intestinal microbiota between aged and adult mice with sepsis.MethodsThirty male C57BL mice were randomly divided into two groups: 15 in the adult group (AD group) and 15 in the age group (Age group). All the mice underwent caecal ligation and puncture to induce sepsis. Mice faeces were collected, and analysed using 16S rRNA sequencing. The liver and colon tissues were collected.ResultsThere were significant differences in intestinal microbiota composition between the two groups. Compared with adult sepsis mice, the diversity of intestinal microbiota in the aged group was significantly reduced and the structure of dominant intestinal microbiota was changed. In the Age group, the microbiota associated with inflammatory factors increased, and the microbiota associated with the production of SCFAs (Ruminiclostridium, Prevotellaceae_UCG-001, Rikenella, Parabacteroides, Oscillibacter, Odoribacter, Muribaculum, Lachnoclostridium, Intestinimonas, Faecalibaculum, Anaerotruncus, Alloprevotella and Absiella) decreased. The metabolic pathways related to the microbiota also changed. Moreover, the proportion of inflammatory factors in Age group was higher than that in AD group.ConclusionOur results showed that there were significant differences in the abundance and structure of microbiota between aged and adult sepsis mice, Aged sepsis mice have more severe intestinal microbiota destruction and liver tissue inflammation than adult sepsis mice.
Subject
Infectious Diseases,Microbiology (medical),Immunology,Microbiology
Reference77 articles.
1. An insight into gut microbiota and its functionalities;Adak;Cell Mol. Life Sci.,2019
2. The gut microbiome's role in the development, maintenance, and outcomes of sepsis;Adelman;Crit. Care,2020
3. Gut microbiota profiles in critically ill patients, potential biomarkers and risk variables for sepsis;Agudelo-Ochoa;Gut. Microbes,2020
4. Collapse of the microbiome, emergence of the pathobiome, and the immunopathology of sepsis;Alverdy;Crit. Care Med.,2017
5. Role of the microbiota in immunity and inflammation;Belkaid;Cell,2014
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献