Author:
Toor Salman M.,Aldous Eman K.,Parray Aijaz,Akhtar Naveed,Al-Sarraj Yasser,Abdelalim Essam M.,Arredouani Abdelilah,El-Agnaf Omar,Thornalley Paul J.,Pananchikkal Sajitha V.,Pir Ghulam Jeelani,Kuni Raheem Ayadathil Thazhhe,Shuaib Ashfaq,Alajez Nehad M.,Albagha Omar M. E.
Abstract
Stroke is the second leading cause of global mortality and continued efforts aim to identify predictive, diagnostic, or prognostic biomarkers to reduce the disease burden. Circulating microRNAs (miRNAs) have emerged as potential biomarkers in stroke. We performed comprehensive circulating miRNA profiling of ischemic stroke patients with or without type 2 diabetes mellitus (T2DM), an important risk factor associated with worse clinical outcomes in stroke. Serum samples were collected within 24 h of acute stroke diagnosis and circulating miRNAs profiled using RNA-Seq were compared between stroke patients with T2DM (SWDM; n = 92) and those without T2DM (SWoDM; n = 98). Our analysis workflow involved random allocation of study cohorts into discovery (n = 96) and validation (n = 94) datasets. Five miRNAs were found to be differentially regulated in SWDM compared to SWoDM patients. Hsa-miR-361-3p and -664a-5p were downregulated, whereas miR-423-3p, -140-5p, and -17-3p were upregulated. We also explored the gene targets of these miRNAs and investigated the downstream pathways associated with them to decipher the potential pathways impacted in stroke with diabetes as comorbidity. Overall, our novel findings provide important insights into the differentially regulated miRNAs, their associated pathways and potential utilization for clinical benefits in ischemic stroke patients with diabetes.
Funder
Qatar Foundation
Qatar National Research Fund
Hamad Medical Corporation
Subject
Cardiology and Cardiovascular Medicine
Reference95 articles.
1. Global, regional, and national burden of stroke and its risk factors, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019.;Lancet Neurol.,2021
2. Heart disease and stroke statistics-2022 update: a report from the American Heart Association.;Tsao;Circulation.,2022
3. An updated definition of stroke for the 21st century: a statement for healthcare professionals from the American Heart Association/American Stroke Association.;Sacco;Stroke.,2013
4. Global, regional, and country-specific lifetime risks of stroke, 1990 and 2016.;Gbd 2016 Lifetime Risk of Stroke Collaborators, Feigin;N Engl J Med.,2018
5. Classification of subtype of acute ischemic stroke. Definitions for use in a multicenter clinical trial. TOAST. Trial of Org 10172 in Acute Stroke Treatment.;Adams;Stroke.,1993
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