The effect of ionising radiation on the phenotype of bone marrow-derived extracellular vesicles

Author:

Kis Dávid1,Persa Eszter1,Szatmári Tünde1,Antal Lilla1,Bóta Attila2,Csordás Ilona Barbara1,Hargitai Rita1,Jezsó Bálint34,Kis Enikő1,Mihály Judith2,Sáfrány Géza1,Varga Zoltán2,Lumniczky Katalin1

Affiliation:

1. Department of Radiobiology and Radiohygiene, National Public Health Center - Radiation Medicine Unit, Budapest, Hungary

2. Research Centre for Natural Sciences - Biological Nanochemistry Research Group, Budapest, Hungary

3. Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary

4. Research Centre for Natural Sciences, Institute of Enzymology, Budapest, Hungary

Abstract

Objectives: Ionising radiation-induced alterations affecting intercellular communication in the bone marrow (BM) contribute to the development of haematological pathologies. Extracellular vesicles (EVs), which are membrane-coated particles released by cells, have important roles in intercellular signalling in the BM. Our objective was to investigate the effects of ionising radiation on the phenotype of BM-derived EVs of total-body irradiated mice. Methods: CBA mice were irradiated with 0.1 Gy or 3 Gy X-rays. BM was isolated from the femur and tibia 24 h after irradiation. EVs were isolated from the BM supernatant. The phenotype of BM cells and EVs was analysed by flow cytometry. Results: The mean size of BM-derived EVs was below 300 nm and was not altered by ionising radiation. Their phenotype was very heterogeneous with EVs carrying either CD29 or CD44 integrins representing the major fraction. High-dose ionising radiation induced a strong rearrangement in the pool of BM-derived EVs which were markedly different from BM cell pool changes. The proportion of CD29 and CD44 integrin-harbouring EVs significantly decreased and the relative proportion of EVs with haematopoietic stem cell or lymphoid progenitor markers increased. Low-dose irradiation had limited effect on EV secretion. Conclusions: Ionising radiation induced selective changes in the secretion of EVs by the different BM cell subpopulations. Advances in knowledge: The novelty of the paper consists of performing a detailed phenotyping of BM-derived EVs after in vivo irradiation of mice.

Publisher

British Institute of Radiology

Subject

Radiology Nuclear Medicine and imaging,General Medicine

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