Genome-Wide Analysis of the DC1 Domain Protein Gene Family in Tomatoes under Abiotic Stress
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Published:2023-11-30
Issue:23
Volume:24
Page:16994
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Li Guobin123ORCID, Dang Jiao123, Pan Jiaqi123, Liu Jingyi1, Peng Tieli123, Chen Guo1, Wang Rongqun1, Hu Songshen123, Li Xiaojing123ORCID, Hu Xiaohui123ORCID
Affiliation:
1. College of Horticulture, Northwest A&F University, Yangling 712100, China 2. Key Laboratory of Protected Horticultural Engineering in Northwest, Ministry of Agriculture, Yangling 712100, China 3. Shaanxi Protected Agriculture Research Centre, Yangling 712100, China
Abstract
DC1 (Divergent C1) domain proteins are a new class of proteins that have been discovered in recent years, which play an important role in plant growth, development, and stress response. In order to better study the distribution and function of DC1 domain proteins in tomatoes, a genome-wide identification was conducted. It was found that there are twenty-one DC1 domain protein genes distributed on nine chromosomes of tomatoes, named SlCHP1-21. Phylogenetic analysis shows that twenty-one SlCHP genes are divided into six subfamilies. Most of the SlCHP genes in tomatoes have no or very short introns. All SlCHP proteins, with the exception of SlCHP8 and SlCHP17, contain variable amounts of C1 domain. Analysis of the SlCHP gene promoter sequence revealed multiple cis-elements responsive to plant stress. qRT-CR analysis showed that most members of SlCHP gene expressed in the roots. The SlCHP11, 13, 16, 17, and SlCHP20 genes showed specific responses to high temperature, low temperature, salt, and drought stress. In addition, the subcellular localization and interaction proteins of SlCHP were analyzed and predicted. Together, these results provides a theoretical basis for further exploration of the function and mechanism of the SlCHP gene in tomatoes.
Funder
Scientific Startup Foundation for Doctors of Northwest A&F University Postdoctoral Research Project of Shaanxi Province
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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