Affiliation:
1. Animal Nutrition and Feed Science, Inner Mongolia Agricultural University, Hohhot 010018, China
2. Food Science, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract
Appropriate soluble carbohydrate (SCHO)-to-NDF ratios in the diet are essential for rumen health. The effects of different SCHO-to-NDF ratios (1.0, 1.5, and 2.0) on rumen barrier function and inflammation in Dumont lambs (n = 18, 6 replicates per treatment) was investigated. The SCHO:NDF ratio was altered by replacing the forage (Leynus chinensis) with corn grain. With an increase in the proportion of SCHO, the final body weight (FBW), average daily gain (ADG), soluble carbohydrate intake (SCHOI), and LPS level increased; and the neutral detergent fiber intake (NDFI), ruminal papillae height, papillae area, and pH decreased (p < 0.05, plin < 0.05). The medium CHO:NDF group had increased claudin-1 mRNA (p < 0.05, plin = 0.005, pquad = 0.003) and protein (p < 0.05, pquad < 0.001) levels; the high CHO:NDF group had increased occludin mRNA and protein (p < 0.05, plin = 0.001) levels. The level of the anti-inflammatory cytokine IL-10 was significantly greater in the medium CHO:NDF group than in the high CHO:NDF group (p < 0.05, pquad < 0.001). With an increase in the ratio of SCHO, the mRNA level and concentration of the proinflammatory cytokines IL-1β, IL-6, and TNF-α linearly increased (p < 0.05, plin < 0.05), and those in the high CHO:NDF group were significantly greater than those in the low CHO:NDF group. The levels of phosphorylated p65 (plin = 0.003), IκB-α (plin < 0.001), and JNK (plin = 0.001) increased linearly, and those in the high CHO:NDF group were significantly greater than those in the other two groups (p < 0.05). Therefore, when the SCHO-to-NDF ratio was increased to 1.5, the rumen epithelium was not affected, but when the ratio was increased to 2.0, NF-κB and MAPK were activated in the rumen epithelium, leading to impaired barrier function and inflammation. The suitable NFC:NDF ratio for the short-term fattening of Dumont lambs was found to be 1.50.
Funder
Research Fund for Universities in Inner Mongolia Autonomous Region
National Natural Science Foundation of China
Natural Science Foundation of Inner Mongolia Autonomous Region
Reference55 articles.
1. Beef species-ruminant nutrition cactus beef symposium: Energy and roughage levels in cattle receiving diets and impacts on health, performance, and immune responses1;Richeson;J. Anim. Sci.,2019
2. Effects of the higher concentrate ratio on the production performance, ruminal fermentation, and morphological structure in male cattle-yaks;Jiang;Vet. Med. Sci.,2022
3. Invited review: Effect of subacute ruminal acidosis on gut health of dairy cows;Plaizier;J. Dairy Sci.,2022
4. An increase in dietary non-structural carbohydrates alters the structure and metabolism of the rumen epithelium in lambs;Steele;Can. J. Plant Sci.,2012
5. Wang, L., Li, Y., Zhang, Y., and Wang, L. (2020). The Effects of Different Concentrate-to-Forage Ratio Diets on Rumen Bacterial Microbiota and the Structures of Holstein Cows During the Feeding Cycle. Animals, 10.
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