CD90 Is Dispensable for White and Beige/Brown Adipocyte Differentiation

Author:

Dahlhaus Meike,Roos JulianORCID,Engel Daniel,Tews Daniel,Halbgebauer Daniel,Funcke Jan-Bernd,Kiener Sophie,Schuler Patrick J.ORCID,Döscher Johannes,Hoffmann Thomas K.,Zinngrebe Julia,Rojewski Markus,Schrezenmeier Hubert,Debatin Klaus-Michael,Wabitsch Martin,Fischer-Posovszky PamelaORCID

Abstract

Brown adipose tissue (BAT) is a thermogenic organ in rodents and humans. In mice, the transplantation of BAT has been successfully used to combat obesity and its comorbidities. While such beneficial properties of BAT are now evident, the developmental and cellular origins of brown, beige, and white adipocytes have remained only poorly understood, especially in humans. We recently discovered that CD90 is highly expressed in stromal cells isolated from human white adipose tissue (WAT) compared to BAT. Here, we studied whether CD90 interferes with brown or white adipogenesis or white adipocyte beiging. We applied flow cytometric sorting of human adipose tissue stromal cells (ASCs), a CRISPR/Cas9 knockout strategy in the human Simpson-Golabi-Behmel syndrome (SGBS) adipocyte model system, as well as a siRNA approach in human approaches supports the hypothesis that CD90 affects brown or white adipogenesis or white adipocyte beiging in humans. Taken together, our findings call the conclusions drawn from previous studies, which claimed a central role of CD90 in adipocyte differentiation, into question.

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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