Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in Brassica napus L. Shoots
Author:
Shi Rui,Pang Chengke,Wu Xu,Zhao Xiaozhen,Chen Feng,Zhang Wei,Sun Chengming,Fu Sanxiong,Hu Maolong,Zhang Jiefu,Wang Xiaodong
Abstract
Background: Brassica napus is one of the most important oil crops in the world, and B. napus shoots are nutrient-rich fresh vegetables. The crude fiber (CF) component is one of the most important factors affecting the taste quality of B. napus shoots, but the factors underlying the desirable low-CF trait remain poorly understood. Methods: In this study, a high-density single-nucleotide polymorphism (SNP) map was used to map quantitative trait loci (QTLs) for five CF-related traits in a recombinant inbred population. Results: A total of 49 QTLs were obtained in four environments, including eleven, twelve, eight, twelve and six QTLs for content of neutral detergent fiber, acid detergent fiber, acid detergent lignin, hemicellulose and cellulose, respectively. The phenotypic variation explained by single QTL ranged from 4.62% to 14.76%. Eight of these QTLs were further integrated into four unique QTLs, which controlled two different traits simultaneously. Five CF-component-related candidate genes were identified, among which BnaC03g07110D and BnaC07g21271D were considered to be the most likely candidate genes. In addition, five lines with low CF content were selected, which can be used as excellent germplasm resources in breeding. Conclusions: The QTLs identified in this study will contribute to our understanding of the genetic mechanism of CF and can be used as targets for reducing CF content in B. napus shoots. In addition, this study also provided excellent germplasm resources for low CF content breeding.
Funder
National Natural Science Foundation of China China Agriculture Research System of MOF and MARA Jiangsu Collaborative Innovation Center for Modern Crop Production
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference54 articles.
1. Exploring the Gene Pool of Brassica napus by Genomics Based Approaches;Hu;Plant Biotechnol. J.,2021 2. Cold-Pressed Rapeseed (Brassica napus) Oil: Chemistry and Functionality;Chew;Food Res. Int.,2020 3. Xiao, Z., Pan, Y., Wang, C., Li, X., Lu, Y., Tian, Z., Kuang, L., Wang, X., Dun, X., and Wang, H. (2022). Multi-Functional Development and Utilization of Rapeseed: Comprehensive Analysis of the Nutritional Value of Rapeseed Sprouts. Foods, 11. 4. New–Demand Oriented Oilseed Rape Industry Developing Strategy;Wang;Chin. J. Oil Crop Sci.,2018 5. Wu, X., Chen, F., Zhao, X., Pang, C., Shi, R., Liu, C., Sun, C., Zhang, W., Wang, X., and Zhang, J. (2021). QTL Mapping and GWAS Reveal the Genetic Mechanism Controlling Soluble Solids Content in Brassica napus Shoots. Foods, 10.
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