Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences

Author:

Wang MengyuORCID,Wang Haoqian,Li Kai,Li Xiaoman,Wang Xujing,Wang Zhixing

Abstract

Nowadays, with the rapid development of biotechnology, the CRISPR/Cas technology in particular has produced many new traits and products. Therefore, rapid and high-resolution detection methods for biotechnology products are urgently needed, which is extremely important for safety regulation. Recently, in addition to being gene editing tools, CRISPR/Cas systems have also been used in detection of various targets. CRISPR/Cas systems can be successfully used to detect nucleic acids, proteins, metal ions and others in combination with a variety of technologies, with great application prospects in the future. However, there are still some challenges need to be addressed. In this review, we will list some detection methods of genetically modified (GM) crops, gene-edited crops and single-nucleotide polymorphisms (SNPs) based on CRISPR/Cas systems, hoping to bring some inspiration or ideas to readers.

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Reference132 articles.

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1. Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize;International Journal of Molecular Sciences;2023-07-31

2. RNA-Dependent RNA Targeting by CRISPR-Cas Systems: Characterizations and Applications;International Journal of Molecular Sciences;2023-04-07

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