A Thermostable Cas12b-Powered Bioassay Coupled with Loop-Mediated Isothermal Amplification in a Customized “One-Pot” Vessel for Visual, Rapid, Sensitive, and On-Site Detection of Genetically Modified Crops

Author:

Han Xiao1,Lu Minghui1,Zhang Yaru1,Liu Xinru1,Zhang Qiang12,Bai Xue3,Man Shuli1,Zhao Liangjuan4,Ma Long1ORCID

Affiliation:

1. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China

2. Branch of Tianjin Third Central Hospital, Tianjin 300457, China

3. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, China

4. Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, China

Funder

Tianjin Municipal Science and Technology Committee

National Natural Science Foundation of China

State Key Laboratory of Food Nutrition and Safety

Tianjin graduate scientific research innovation project

Publisher

American Chemical Society (ACS)

Reference50 articles.

1. International Service for the Acquisition of Agri-biotech Applications (ISAAA). Global status of commercialized biotech/GM crops in 2019: biotech crops drive socio-economic development and sustainable environment in the new frontier. Available online: https://www.isaaa.org/resources/publications/briefs/55/default.asp (accessed on 8 December 2022).

2. Genetically modified organisms and food security in Southern Africa: conundrum and discourse

3. How Can We Better Detect Unauthorized GMOs in Food and Feed Chains?

4. The Chinese public’s awareness and attitudes toward genetically modified foods with different labeling

5. Recent advances in emerging DNA-based methods for genetically modified organisms (GMOs) rapid detection

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