The Effects of Ozone on Herbivore-Induced Volatile Emissions of Cultivated and Wild Brassica Rapa

Abstract

Since preindustrial times, concentrations of tropospheric ozone, a phytotoxic pollutant, have risen in the Northern Hemisphere. Selective breeding has intentionally modified crop plant traits to improve yield but may have altered plant defenses against abiotic and biotic stresses. This study aims to determine if cultivated and wild plants respond differently to herbivory under elevated ozone. We studied the volatile emissions of four cultivated Brassica rapa ssp. oleifera varieties and one wild population after exposure to ozone or Plutella xylostella larval feeding either individually or together. Ozone modulated the volatiles emitted in response to herbivory by all plant varieties to different extents. We did not observe a clear difference in the effects of ozone on wild and cultivated plants, but cultivated plants had higher volatile emission rates in response to herbivory and ozone had either no effect or increased the herbivore-induced response. Larvae tended to feed more on elevated ozone-treated plants; however, we could not link the increase of feeding to the change in volatile emissions. Our study complements recent studies reporting that selective breeding might not have weakened chemical defenses to biotic and abiotic stresses of cultivated plants.