Development of a Dual Mode UCNPs-MB Biosensor in Combination with PCR for Sensitive Detection of Salmonella

Author:

Han Lu1,Chen Min1,Song Yaqi1,Yan Zhongyu1,Zhou Dandan2,Pan Leiqing1ORCID,Tu Kang1

Affiliation:

1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

2. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China

Abstract

In recent years, the high prevalence of Salmonella has emerged as a serious threat to public safety, prompting attempts to utilize accurate, rapid, and direct methods to ensure food safety. In this study, a multifunctional platform featuring dual-mode detection channels (colorimetric-fluorescence) combined with polymer chain reaction (PCR) was proposed for the sensitive and rapid detection of Salmonella. Additionally, the colorimetric measurements were achieved by color changes induced by methylene blue (MB) insertion into the double-stranded DNA, and the fluorescence measurements were performed by internal filter effect (IFE)-induced fluorescence quenching of upconversion nanoparticles (UCNPs) by MB. The results showed that the IFE and PCR amplification processes improved the sensitivity of the sensor towards Salmonella detection, with a limit of detection (LOD) of 21.8 CFU/mL. Moreover, this colorimetric-fluorescence dual-mode PCR biosensor was applied to determine Salmonella in food samples, such as chicken, egg, and fish, which produced satisfactory results. Overall, the present study results demonstrate the potential for combining PCR amplification with IFE to develop an efficient and reliable dual-mode analysis platform to safeguard food security.

Funder

Jiangsu Provincial Key Research and Development Program

Jiangsu Province Graduate Research and Innovation Program

Science and Technology Project of Jiangsu Provincial Market Supervision Administration

Natural Science Research Plan of Huai’an

National Key R&D Program of China

Publisher

MDPI AG

Subject

Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)

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