Optimization of Fermentation Process of Wheat Germ Protein by Aspergillus niger and Analysis of Antioxidant Activity of Peptide

Author:

Liu Yingying1,Zhou Yu1,Zhu Chaohong1,Meng Yanglin1,Wang Jingjing1,Chen Xinyang1,Hou Yinchen2ORCID,Liao Aimei1,Pan Long1,Huang Jihong1234ORCID

Affiliation:

1. College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China

2. Food Laboratory of Zhongyuan, Luohe 462000, China

3. State Key Laboratory of Crop Stress Adaptation and Improvement, College of Agriculture, Henan University, Kaifeng 475004, China

4. School of Food and Pharmacy, Xuchang University, Xuchang 461000, China

Abstract

Utilizing wheat embryos as the raw material and employing Aspergillus niger as the fermentation strain, wheat embryo polypeptides were produced through microbial liquid fermentation. The protein concentration post-fermentation served as the response variable, and the preparation process underwent optimization through single-factor testing and a response surface methodology, followed by the assessment of antioxidant activity. The findings revealed that the optimal conditions for wheat embryo peptide preparation via Aspergillus niger fermentation included a fermentation duration of 24 h, an inoculum volume of 4%, an initial pH of 7, and a protein concentration of 21.47 mg/mL. Peptides with different molecular weights were then prepared by dead-end filtration. The results showed that F6 (<3 kDa) had strong scavenging ability against DPPH, ABTS, and ·OH radicals, which provided a basis for the preparation of antioxidant peptides in wheat germ and related research.

Funder

Cultivation Programme for Young Backbone Teachers in Henan University of Technology, High-Level Talents Research Fund of HAUT

Henan Province Science and Technology Research and Development Plan Joint Fund Project

Henan Province Youth Science Fund Project

Key Research and Development Project of Henan Province

Open Competition Research Projects of Xuchang University

Zhongyuan Scholars of Henan Province in China

Zhongyuan Scholar Workstation Funded Project

Central Government Guides the Local Science and Technology Development Special Fund

Publisher

MDPI AG

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