Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by Actinobacillus succinogenes GXAS137
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Published:2023-12-27
Issue:1
Volume:10
Page:22
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ISSN:2311-5637
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Container-title:Fermentation
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language:en
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Short-container-title:Fermentation
Author:
Qin Yan1, Li Yi1, Liang Ge1, Shen Naikun2, Xian Liang1, Wang Qingyan1
Affiliation:
1. National Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, China 2. School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China
Abstract
Sugarcane bagasse (SCB) is an abundant agricultural waste, rich in cellulose and hemicellulose, that could be used as an ideal raw material for succinic acid (SA) production. A two-step chemical pretreatment, involving alkali extraction and alkaline hydrogen peroxide treatment, was utilized to treat SCB, followed by multi-enzyme hydrolysis to obtain a reducing sugar hydrolysate mainly composed of glucose and xylose. Optimization of the multi-enzyme hydrolysis of pretreated SCB resulted in a final reducing sugar concentration of 78.34 g/L. In order to enhance the bioconversion of SCB to SA and to reduce the production costs, the initial reducing sugar concentration, nitrogen source, and MgCO3 content were further optimized. The results demonstrated that the inexpensive corn steep liquor powder (CSLP) could be utilized as an alternative nitrogen source to yeast extract for the production of SA; and the optimal concentrations of initial reducing sugar, CSLP, and MgCO3 were 70 g/L, 18 g/L, and 60 g/L, respectively. When fed-batch fermentation was conducted in a 2 L stirred bioreactor, approximately 72.9 g/L of SA was produced, with a yield of 83.2% and a productivity of 1.40 g/L/h. The high SA concentration, yield, and productivity achieved in this study demonstrate the potential of SCB, an agricultural waste, as a viable alternative substrate for Actinobacillus succinogenes GXAS137 to produce SA. This lays a solid foundation for the resource utilization of agricultural waste and cost-effective industrial-scale production of SA in the future.
Funder
Natural Science Foundation of Guangxi Province Science and Technology Program of Guangxi Province National Natural Science Foundation of China
Subject
Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science
Reference50 articles.
1. Escanciano, I.A., Ripoll, V., Ladero, M., and Santos, V.E. (2023). Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling. Fermentation, 9. 2. Production of succinic acid by metabolically engineered microorganisms;Ahn;Curr. Opin. Biotechnol.,2016 3. Co-fermentation of glucose and xylose from sugarcane bagasse into succinic acid by Yarrowia lipolytica;Ong;Biochem. Eng. J.,2019 4. Xu, C., Alam, M.A., Wang, Z., Peng, Y., Xie, C., Gong, W., Yang, Q., Huang, S., Zhuang, W., and Xu, J. (2021). Co-fermentation of succinic acid and ethanol from sugarcane bagasse based on full hexose and pentose utilization and carbon dioxide reduction. Bioresour. Technol., 339. 5. Bio-based succinic acid: An overview of strain development, substrate utilization, and downstream purification;Dai;Biofuels Bioprod. Biorefining,2020
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