Genome-Wide Association Study of Egg Production Traits in Shuanglian Chickens Using Whole Genome Sequencing
Author:
Fu Ming12, Wu Yan12, Shen Jie12, Pan Ailuan12, Zhang Hao12, Sun Jing12, Liang Zhenhua12, Huang Tao12, Du Jinping12, Pi Jinsong12
Affiliation:
1. Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science, Wuhan 430064, China 2. Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Hubei Academy of Agricultural Science, Wuhan 430064, China
Abstract
Egg production is the most important economic trait in laying hens. To identify molecular markers and candidate genes associated with egg production traits, such as age at first egg (AFE), weight at first egg (WFE), egg weight (EW), egg number (EN), and maximum consecutive egg laying days (MCD), a genome-wide analysis by whole genome sequencing was performed in Shuanglian chickens. Through whole genome sequencing and quality control, a total of 11,006,178 SNPs were obtained for further analysis. Heritability estimates ranged from moderate to high for EW (0.897) and MCD (0.632), and from low to moderate (0.193~0.379) for AFE, WFE, and EN. The GWAS results showed 11 genome-wide significant SNPs and 23 suggestive significant SNPs were identified to be associated with EN, MCD, WFE, and EW. Linkage disequilibrium analysis revealed twenty-seven SNPs associated with EN were located in a 0.57 Mb region on GGA10, and clustered into five blocks. Through functional annotation, three candidate genes NEO1, ADPGK, and CYP11A1, were identified to be associated with EN, while the S1PR4, LDB2, and GRM8 genes was linked to MCD, WFE, and EW, respectively. These findings may help us to better understand the molecular mechanisms underlying egg production traits in chickens and contribute to genetic improvement of these traits.
Funder
Key Research and Development Plan Projects of Hubei Province Hubei Academy of Agricultural Sciences Youth Science Foundation Project Hubei Provincial Science and Technology Innovation Team Project
Subject
Genetics (clinical),Genetics
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