Abstract
Haemagogus (Haemagogus) janthinomys (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F1 generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F3 generation Hg. janthinomys eggs for the first time. A total of 62.8% (1562/2486) F1 generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F2 generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F2 mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of Hg. janthinomys past the F3 generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.
Funder
International Collaborations in Infectious Disease Research
Centers for Research in Emerging Infectious Diseases
National Institutes of Health
Consejo Nacional de Ciencia y Tecnología
Subject
Virology,Infectious Diseases
Reference61 articles.
1. Yellow fever virus in jungle mosquitoes;Shannon;Science,1938
2. Mosquito studies (Diptera, Culicidae) XXXII: A revision of the genus Haemagogus;Arnell;Contrib. Am. Entomol. Inst.,1973
3. Pinheiro, G.G., Rocha, M.N., de Oliveira, M.A., Moreira, L.A., and Andrade Filho, J.D. (2019). Detection of yellow fever virus in sylvatic mosquitoes during disease outbreaks of 2017–2018 in Minas Gerais State, Brazil. Insects, 10.
4. Haemagogus leucocelaenus and Haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in Brazil, 2016–2018;Abreu;Emerg. Microbes Infect.,2019
5. GloPID-R report on chikungunya, o’nyong-nyong and Mayaro virus, part 5: Entomological aspects;Pezzi;Antiviral Res.,2020
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献