Affiliation:
1. Interdisciplinary Centre of Marine and Environmental Research (CIIMAR-UP), 4450-208 Porto, Portugal
2. Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal
3. Centre of Biological Engineering, University of Minho, 4704-553 Braga, Portugal
Abstract
This study aimed to evaluate the effects of dietary inclusion of plant feedstuff mixture (PFM) pre-treated by solid-state fermentation (SSF) on the physiological responses of European seabass. For that purpose, two diets were formulated to contain: 20% inclusion level of non-fermented plant ingredients mixture (20Mix) and 20Mix fermented by A. niger in SSF conditions (20Mix-SSF). Seabass juveniles (initial body weight: 20.9 ± 3.3 g) were fed the experimental diets, reared at two different temperatures (21 and 26 °C) and subjected to weekly salinity oscillations for six weeks. Growth performance, digestive enzyme activities, humoral immune parameters, and oxidative stress indicators were evaluated. A reduction in weight gain, feed intake, and thermal growth coefficient was observed in fish fed the fermented diet (20Mix-SSF). Salinity oscillation led to an increase in weight gain, feed efficiency, daily growth index, and thermal growth coefficient, regardless of dietary treatment. Higher rearing temperatures also increased daily growth index. No dietary effect was observed on digestive enzymes activities, whereas rearing temperature and salinity oscillation modulated digestive enzyme activities. Oxidative stress responses were significantly affected by experimental diets, temperature, and salinity conditions. Catalase and glutathione peroxidase activities showed an interactive effect. Fish reared at 21 °C showed higher enzymatic activity when fed the 20Mix-SSF. Conversely, fish reared at 26 °C showed higher GPx activity when fed the 20Mix diet. Fish reared at 26 °C showed reduced peroxidase and lysozyme activities, while salinity fluctuation led to increased lysozyme activity and decreased ACH50 activity. ACH50 activity increased in fish fed the 20Mix-SSF. Overall, the dietary inclusion of PFM fermented by A. niger was unable to mitigate the impact of environmental stress on physiological performance in European seabass. In fact, fermented feed caused an inhibition of growth performances and an alteration of some physiological stress indicators.
Funder
Ocean3R project
North Portugal Regional Operational Program
PORTUGAL 2020 Partnership Agreement
European Regional Development Fund
FCT—Foundation for Science and Technology
Subject
General Veterinary,Animal Science and Zoology
Reference119 articles.
1. IPCC (2022). Climate Change 2022: Impacts, Adaptation and Vulnerability.
2. Improved Estimates of Changes in Upper Ocean Salinity and the Hydrological Cycle;Cheng;J. Clim.,2020
3. Barange, M., Bahri, T., Beveridge, M., Cochrane, K., Funge-Smith, S., and Poulain, F. (2018). Impacts of Climate Change on Fisheries and Aquaculture. Synthesis of Current Knowledge, Adaptation, and Mitigation Options, Food and Agriculture Organization.
4. Brander, K., Cochrane, K., Barange, M., and Soto, D. (2017). Climate Change Impacts on Fisheries and Aquaculture, Organization of the United Nations.
5. Behavioral Thermoregulation and the “Final Preferendum” Paradigm;Reynolds;Am. Zool.,2015
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