The Effect of Silencing Fatty Acid Elongase 4 and 6 Genes on the Proliferation and Migration of Colorectal Cancer Cells-Reference-Cited by-同舟云学术

The Effect of Silencing Fatty Acid Elongase 4 and 6 Genes on the Proliferation and Migration of Colorectal Cancer Cells

Published:2023-12-18 Issue:24 Volume:24 Page:17615
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Czumaj Aleksandra¹^ORCID,Kobiela Jarosław²³,Mika Adriana¹^ORCID,Pappou Emmanouil⁴^ORCID,Śledziński Tomasz¹^ORCID

Affiliation:

1. Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-211 Gdansk, Poland

2. Department of General, Endocrine and Transplant Surgery, Faculty of Medicine, Medical University of Gdansk, 80-211 Gdansk, Poland

3. Department of Surgical Oncology, Faculty of Medicine, Medical University of Gdansk, 80-211 Gdansk, Poland

4. Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA

Abstract

Colorectal cancer (CRC) cells show some alterations in lipid metabolism, including an increased fatty acid elongation. This study was focused on investigating the effect of a small interfering RNA (siRNA)-mediated decrease in fatty acid elongation on CRC cells’ survival and migration. In our study, the elongase 4 (ELOVL4) and elongase 6 (ELOVL6) genes were observed to be highly overexpressed in both the CRC tissue obtained from patients and the CRC cells cultured in vitro (HT-29 and WiDr cell lines). The use of the siRNAs for ELOVL4 and ELOVL6 reduced cancer cell proliferation and migration rates. These findings indicate that the altered elongation process decreased the survival of CRC cells, and in the future, fatty acid elongases can be potentially good targets in novel CRC therapy.

Funder

National Science Centre of Poland

Medical University of Gdansk Young Creator of Science Program

Medical University of Gdansk

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Link

https://www.mdpi.com/1422-0067/24/24/17615/pdf

Reference45 articles.

1. Colon Cancer: A Clinician’s Perspective in 2019;Ahmed;Gastroenterol. Res.,2020

2. Malki, A., Elruz, R.A., Gupta, I., Allouch, A., Vranic, S., and Al Moustafa, A.E. (2021). Molecular mechanisms of colon cancer progression and metastasis: Recent insights and advancements. Int. J. Mol. Sci., 22.

3. Lipid metabolism and cancer;Bian;J. Exp. Med.,2021

4. Lipid mechanisms in hallmarks of cancer;Molendijk;Mol. Omics,2020

5. Salita, T., Rustam, Y.H., Mouradov, D., Sieber, O.M., and Reid, G.E. (2022). Reprogrammed Lipid Metabolism and the Lipid-Associated Hallmarks of Colorectal Cancer. Cancers, 14.