Affiliation:
1. Faculty of Food Science and Engineering, Dunărea de Jos University of Galati, 111 Domnească Street, 800201 Galati, Romania
Abstract
Using a Central Composite Design, the extraction of bioactive compounds from red grape Băbească neagră peels was optimized by applying a conventional solvent extraction. On the anthocyanin content, total phenolic content, and antioxidant activity (using the DPPH method), the effects of extraction factors, including ethanol and citric acid concentrations, extraction temperature, and duration, were investigated. For each of the investigated parameters, a quadratic model was suggested. The maximum and minimum variables investigated in the coded form of the experimental plan are the concentrations of citric acid (0.10–2.64%), ethanol (38.06–96.93%), operating temperature (13.06–71.90 °C), and extraction time (11.36–78.63 min). The optimal mixture for recovering the most significant amount of polyphenol content and antioxidant activity was 85% ethanol, 0.85% citric acid, 52.14 min, and 57 °C. Based on the experimental approach, the anthocyanin content ranged from 1.71 to 2.74 mg C3G/g DW, the total phenolic content ranged from 24.67 to 43.97 mg/g, and the antioxidant activity ranged from 15.95 to 20.98 mM TE/g DW. Overall, it should be stressed that establishing operating factors to maximize model responses can improve the extraction process and the obtaining of red grape peel value-added extracts for creating functional food products.
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