Author:
Balkrishna ,Das ,Pokhrel ,Joshi ,Laxmi ,Verma ,Sharma ,Sharma ,Sharma ,Joshi
Abstract
Colchicine was extracted from Gloriosa superba seeds using the Super Critical Fluid (CO2) Extraction (SCFE) technology. The seeds were purified upto 99.82% using column chromatography. Colchicine affinity was further investigated for anticancer activity in six human cancer cell lines, i.e., A549, MCF-7, MDA-MB231, PANC-1, HCT116, and SiHa. Purified colchicine showed the least cell cytotoxicity and antiproliferation and caused no G2/M arrest at clinically acceptable concentrations. Mitotic arrest was observed in only A549 and MDA-MB231 cell lines at 60nM concentration. Our finding indicated the possible use of colchicine at a clinically acceptable dose and provided insight into the science behind microtubule destabilization. However, more studies need to be conducted beforethese findings could be established.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference50 articles.
1. The Useful Plants of India;Ambasta,1986
2. Indian Materia Medica, Vol. I.–II;Nadkarni,1976
3. Medicinal plants in India, vol. I;Pulliah,2002
4. Tropolonic colchicum alkaloids. The Alkaloids: Chemistry and Pharmacology;Capraro,1984
5. Colchicine Significantly Reduces Incident Cancer in Gout Male Patients
Cited by
24 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献