Interactions between Sugarcane Leaf Return and Fertilizer Reduction in Soil Bacterial Network in Southern China Red Soil

Abstract

Microbes may play an important role in the sugarcane leaf degradation and nutrient conversion process. Soil bacterial communities are more or less involved in material transformation and nutrient turnover. In order to make better use of the vast sugarcane leaf straw resources and reduce the overuse of chemical fertilizers in the subtropical red soil region of Guangxi, a pot experiment, with three sugarcane leaf return (SLR) amounts [full SLR (FS), 120 g/pot; half SLR (HS), 60 g/pot; and no SLR (NS)] and three fertilizer reduction (FR) levels [full fertilizer (FF), 4.50 g N/pot, 3.00 g P2O5/pot, and 4.50 g K2O/pot; half fertilizer (HF), 2.25 g N/pot, 1.50 g P2O5/pot, and 2.25 g K2O/pot; and no fertilizer (NF)], was conducted to assess the interactions of different SLR amounts and chemical FR levels in the soil bacterial network and the relationship between the soil properties and bacterial network by using Illumina Miseq high-throughput sequencing technology. According to the results of the soil bacterial community compositions and diversity, the soil bacterial network was changed during maize growth. SLR exerted a stronger effect on soil bacterial function than FR. Returning the sugarcane leaf to the field increased the diversity of the soil bacteria network. The bacterial communities were consistently dominated by Acidobacteria, Actinobacteria, and Bacteroidetes across all treatments, among which Actinobacteria was the most abundant bacteria type by almost 50% at the phylum level. The analysis results of the experimental factor on maize growth showed that the effect of SLR was lower than that of FR; however, this was opposite in the soil bacterial community structure and diversity. The soil bacterial network was significantly correlated with the soil total K, available N and organic matter contents, and EC. The soil bacteria community showed different responses to SLR and FR, and the FF in combination with FS partly increased the complexity of the soil bacteria network, which can further benefit crop production and soil health in the red soil region.