Fusarium Species Causing Pepper Wilt in Russia: Molecular Identification and Pathogenicity
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Published:2024-02-06
Issue:2
Volume:12
Page:343
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Engalycheva Irina1ORCID, Kozar Elena1, Frolova Svetlana1, Vetrova Svetlana1, Tikhonova Tatyana1, Dzhos Elena1, Engalychev Myazar1, Chizhik Vera12, Martynov Viktor2, Shingaliev Andrey2ORCID, Dudnikova Ksenia23ORCID, Dudnikov Maksim2, Kostanchuk Yulia4
Affiliation:
1. Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC), 143072 Vniissok, Russia 2. State Budgetary Scientific Institution Federal, All-Russian Research Institute of Agricultural Biotechnology (FSBSI ARRIAB), 127550 Moscow, Russia 3. Federal Research Center of Biological Plant Protection, 350039 Krasnodar, Russia 4. Federal State Budget Scientific Institution, Research Institute of Agriculture of Crimea, 295034 Simferopol, Russia
Abstract
Fusarium wilt pathogens represent an ongoing threat to pepper production worldwide. This is the first report providing data on the molecular identification of Fusarium fungi that cause wilt in pepper in the southern regions of Russia. Monitoring of the Fusarium infection on pepper was carried out in 2019–2022 in two economically important regions of this culture production: the Krasnodar Krai and Crimea. Based on a phylogenetic analysis of the translation elongation factor (EF1a) and the internal transcribed spacer (ITS), as well as the macro- and micromorphological characteristics of the fungi, the causative agents of Fusarium wilt have been identified. The causative agents identified as representatives of the Fusarium species composition included: F. clavus, F. solani, F. oxysporum, F. verticillioides, F. commune, F. torulosum, and F. sporotrichioides. Depending on the region, the specifics of biodiversity and the ratio of these species in pathocomplexes were noted. In Crimea, wilting could be attributed to all of the identified species; in the Krasnodar Krai, F. verticillioides and F. clavus were found to contribute to wilting. The pathogenicity test showed that the pathogens of pepper wilting in Russia, in addition to the already known F. oxysporum and F. solani, are the species F. clavus and F. verticillioides. This is the first report on the ability of these species to cause Fusarium wilt in pepper cultures. The obtained data will be of practical value for the development of biological control measures for fungi of the genus Fusarium, which cause pepper wilt in areas of industrial production and seed production. In addition, data on species composition and aggressive isolates will be used in a pepper breeding program for resistance to Fusarium wilt.
Subject
Virology,Microbiology (medical),Microbiology
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