Affiliation:
1. Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University of Sichuan Province, Chengdu 611130, China
2. College of Forestry, Sichuan Agricultural University, Chengdu 611130, China
Abstract
As a kind of plant-specific transcription factor (TF), DNA-Binding One Zinc Finger (Dof) is widely involved in the response to environmental change, and as an evolutionarily important perennial plant species, Akebia trifoliata is ideal for studying environmental adaptation. In this study, a total of 41 AktDofs were identified in the A. trifoliata genome. First, the characteristics, including the length, exon number, and chromosomal distribution of the AktDofs and the isoelectric point (PI), amino acid number, molecular weight (MW), and conserved motifs of their putative proteins, were reported. Second, we found that all AktDofs evolutionarily underwent strong purifying selection, and many (33, 80.5%) of them were generated by whole-genome duplication (WGD). Third, we outlined their expression profiles by the use of available transcriptomic data and RT-qPCR analysis. Finally, we identified four candidate genes (AktDof21, AktDof20, AktDof36, and AktDof17) and three other candidate genes (AktDof26, AktDof16, and AktDof12) that respond to long day (LD) and darkness, respectively, and that are closely associated with phytohormone-regulating pathways. Overall, this research is the first to identify and characterize the AktDofs family and is very helpful for further research on A. trifoliata adaptation to environmental factors, especially photoperiod changes.
Funder
Sichuan Science and Technology Program
Ya’an and Sichuan Agricultural University
Ya’an Science and Technology Bureau, Shimian County Science and Technology Bureau in Sichuan Province, China
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference40 articles.
1. Plant drought stress: Effects, mechanisms and management;Farooq;Agron. Sustain. Dev.,2009
2. Plants compared to animals: The broadest comparative study of development;Meyerowitz;Science,2002
3. The lateral organ boundaries gene defines a novel, plant-specific gene family;Shuai;Plant Physiol.,2002
4. Plant transcription factors;Meshi;Plant Cell Physiol.,1995
5. The plant-specific dof transcription factors family: New players involved in vascular system development and functioning in Arabidopsis;Bellini;Front. Plant Sci.,2013
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献