Overexpression of NB-LRR Gene AtRPM1(D505V) Improved Drought and Salt Resistance and Decreased Cold Tolerance in Transgenic Rice
Author:
Li Zhaowu1, Zhou Xiaojie2ORCID, Liu Xiaoxiao3, Wu Xiaoqiu1, He Zhiming1, Gao Zhiyong3, Wang Zhangying3
Affiliation:
1. Puai Medical College, Shaoyang University, Shaoyang 422000, China 2. College of Food and Chemical Engineering, Shaoyang University, Shaoyang 422000, China 3. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China
Abstract
Abiotic stimuli severely restrict the growth and development of plants, resulting in massive losses in the quality and yield of crops. Exploring genes that can improve crop tolerance to abiotic stress is important. In a previous study, we found that overexpression of the Arabidopsis nucleotide-binding domain leucine-rich repeat (NB-LRR) gene AtRPM1(D505V) increased disease resistance in rice. In this research, we found that AtRPM1(D505V) transgenic plants were more sensitive to abscisic acid (ABA) than wild type (WT) plants. Abiotic-stress resistance in AtRPM1(D505V) transgenic plants was investigated. We found that AtRPM1(D505V) transgenic plants exhibited improved resistance to drought and salt stress; the phonotype and survival rates of transgenic rice were better than WT plants. The expression of stress responsive genes including OsDREB2A, OsDREB2B, OsRD22, and OsRD29A were significantly upregulated in AtRPM1(D505V) overexpressed plants than in WT plants. Moreover, the activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) were significantly increased in AtRPM1(D505V) overexpressed plants than in WT plants under drought and salt stress. Under cold stress, the expression of stress responsive genes and the activities of antioxidant enzymes in AtRPM1(D505V) transgenic plants were significantly lower than in WT plants. Our research demonstrated that AtRPM1(D505V) confers drought and salt resistance to transgenic rice. Therefore, AtRPM1(D505V) could act as a potential candidate gene to cultivate drought- and salt-tolerant plants.
Funder
National Nature Science Foundation of China The National Key Research and Development Program of China
Reference54 articles.
1. The physiology of plant responses to drought;Gupta;Science,2020 2. Oladosu, Y., Rafii, M.Y., Samuel, C., Fatai, A., Magaji, U., Kareem, I., Kamarudin, Z.S., Muhammad, I., and Kolapo, K. (2019). Drought Resistance in Rice from Conventional to Molecular Breeding: A Review. Int. J. Mol. Sci., 20. 3. Salt tolerance in rice: Physiological responses and molecular mechanisms;Liu;Crop J.,2022 4. Sarma, B., Kashtoh, H., Lama Tamang, T., Bhattacharyya, P.N., Mohanta, Y.K., and Baek, K.-H. (2023). Abiotic Stress in Rice: Visiting the Physiological Response and Its Tolerance Mechanisms. Plants, 12. 5. Zhu, Y., Chen, K., Mi, X., Chen, T., Ali, J., Ye, G., Xu, J., and Li, Z. (2016). Identification and Fine Mapping of a Stably Expressed QTL for Cold Tolerance at the Booting Stage Using an Interconnected Breeding Population in Rice. PLoS ONE, 10.
|
|