Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions
Author:
Tao Qibo123, Xing Jiayi123, Meng Fansheng123, Zhang Yaqi123, Liu Xinyu123, Guo Shuo123, Shan Ye123, Zhong Shangzhi123, Sun Juan123, Zhao Yanhua123
Affiliation:
1. College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China 2. Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China 3. Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, Qingdao 266109, China
Abstract
Seed vigor is an important aspect of seed quality. It is critical to predict seed vigor and plant seedling emergence under diverse environmental conditions using the laboratory vigor test. Accordingly, laboratory experiments were conducted to determine the standard germination (SG), early individual counts of radicle emergence (RE), mean germination time (MGT), and vigor index (VI) for 16 commercially available seed lots of Siberian wildrye (Elymus sibiricus), an economically and ecological important grass species. The field emergence (FE) for seed lots was explored using a three-year field trial from 2021 to 2023. Meanwhile, pot experiments were carried out to determine the seedling emergence performance under different environmental conditions, including control, drought, and salinity stress. The correlation and regression analysis were done to investigate the links between laboratory vigor test with emergence performance under both field and pot conditions. The results showed that the SG of 16 seed lots were high, similar, and did not differ significantly between seed lots. However, the seedling emergence performance (emergence percentage, seedling dry weight, and simplified vigor index) differed significantly between seed lots under both field and pot conditions. The SG was not significantly correlated with seedling emergence performance under either the field or pot conditions. The counts of RE at selected timing, MGT, and VI showed a significant relationship with seed vigor as reflected by seedling emergence performance under diverse environmental conditions, and we found that RE at 108 h was highly predictive of seed vigor and seedling emergence. The RE at 108 h and VI were positively related to seed vigor, while MGT was negatively related. The seed lots with low vigor had lower RE at 108 h and VI but longer MGT. Overall, both RE at 108 h, MGT, and VI can be used to estimate seed vigor and predict seedling emergence under different environmental conditions, and we highly recommend RE at 108 h as a quick, precise, and convenient vigor test and early warning sign for seed storage of E. sibiricus. These results will enable seed researchers, plant breeders, farmers, and government program directors to target higher seed vigor more effectively for E. sibiricus and similar grass species.
Funder
China Agricultural Research System Natural Science Foundation of Shandong Province Qingdao Agricultural University Doctoral Start-up Fund Development Project of Intelligent Weed Identification Technology
Subject
Agronomy and Crop Science
Reference72 articles.
1. Ntakirutimana, F., Xiao, B.W., Xie, W.G., Zhang, J.C., Zhang, Z.Y., Wang, N., and Yan, J.J. (2019). Potential effects of awn length variation on seed yield and components, seed dispersal and germination performance in Siberian wildrye (Elymus sibiricus L.). Plants, 8. 2. Influence of row spacing and P and N applications on seed yield components and seed yield of Siberian wildrye (Elymus sibiricus L.);Wang;Crop Sci.,2017 3. Zheng, Y.Y., Zhang, Z.Y., Wan, Y.Y., Tian, J.Y., and Xie, W.G. (2020). Development of EST-SSR markers linked to flowering candidate genes in Elymus sibiricus L. based on RNA sequencing. Plants, 9. 4. Zhang, J.C., Xie, W.G., Yu, X.X., Zhang, Z.Y., Zhao, Y.Q., Wang, N., and Wang, Y.R. (2019). Selection of suitable reference genes for RT-qPCR gene expression analysis in Siberian Wild Rye (Elymus sibiricus) under different experimental conditions. Genes, 10. 5. The response of soil organic carbon and nitrogen 10 years after returning cultivated alpine steppe to grassland by abandonment or reseeding;Shang;Catena,2014
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