Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis

Abstract

Drought stress critically endangers the growth and development of crops. Glutathione S-transferase plays a vital role in response to abiotic stress. However, there are few studies on the role of glutathione S-transferase in maize drought stress. In this study, the significantly downregulated expression of ZmGST26 in roots under drought stress was analyzed by qRT-PCR. Promoter analyses showed that there were several cis-acting elements related to drought stress and that were involved in oxidative response in the promoter region of ZmGST26. Subcellular localization results showed that ZmGST26 was localized in the nucleus. The transgenic lines of the Arabidopsis over-expressing ZmGST26 were more sensitive to drought stress and ABA in seed germination and inhibited ABA-mediated stomatal closure. Under drought stress, phenotypic analyses showed that the germination rate, root length and survival rate of ZmGST26 overexpressing lines were significantly lower than those of wild-type lines. The determination of physiological and biochemical indexes showed that the water loss rate, malondialdehyde, O2− and H2O2 of the overexpression lines significantly increased compared with wild-type Arabidopsis, but the antioxidant enzyme activities (CAT, SOD and POD), and proline and chlorophyll contents were significantly reduced. Subsequently, the qRT-PCR analysis of drought stress-related gene expression showed that, under drought stress conditions, the expression levels of DREB2A, RD29A, RD29B and PP2CA genes in ZmGST26 overexpression lines were significantly lower than those in wild-type Arabidopsis. In summary, ZmGST26 reduced the drought resistance of plants by aggravating the accumulation of reactive oxygen species in Arabidopsis.