Effects of Sound Wave and Water Management on Growth and Cd Accumulation by Water Spinach (Ipomoea aquatica Forsk.)

Abstract

Vegetable contamination by cadmium (Cd) is of great concern. Water spinach (Ipomoea aquatica) is a common leafy vegetable in many countries and has a strong ability to accumulate Cd. The work was conducted to study the effects of sound wave, water management, and their combination on Cd accumulation and growth of water spinach, using the following three experiments: a hydroponic trial with the treatment of a plant acoustic frequency technology (PAFT) generator in test sheds, a hydroponic trial with three music treatments (electronic music (EM), rock music (RM), and classical music (CM)) in artificial climate boxes, and a soil pot trial with treatments of PAFT and EM under non-flooded and flooded conditions. The results showed that the hydroponic treatments of PAFT and EM significantly reduced the Cd concentrations in roots and shoots (edible parts) of water spinach by 22.01–36.50% compared with the control, possibly due to sound waves decreasing the root tip number per unit area and increasing average root diameter, root surface area, and total root length. Sound wave treatments clearly enhanced water spinach biomass by 28.27–38.32% in the hydroponic experiments. In the soil experiment, the flooded treatment significantly reduced the Cd concentrations in roots and shoots by 43.75–63.75%, compared with the non-flooded treatment. The Cd decrease and the biomass increase were further driven by the PAFT supplement under the flooding condition, likely related to the alteration in root porosity, rates of radial oxygen loss, extractable soil Cd, soil Eh, and soil pH. Our results indicate that the co-application of plant acoustic frequency technology and flooded management may be an effective approach to reduce Cd accumulation in water spinach.