HvWOX3A Gene Controls Plant Height and Leaf Size in Barley

Abstract

Plant height and leaf morphology are considered complex agronomy traits; both are significantly related to nutrient utilization, lodging resistance, and photosynthetic efficiency, which ultimately affect biomass and yield. However, the molecular mechanism of leaf morphogenesis is still unclear. WOX is a group of plant-specific transcription factor families that regulate growth and development, cell division, and differentiation in plants. In the present study, we identified and characterized the m-876 mutant in barley (Hordeum vulgare L.), which exhibited an extreme reduction in leaf width and plant height. Using a map-based cloning strategy, the m-876 mutant was narrowed down to an 11.4 Mb genomic interval on the long arm of chromosome 5. By analyzing the gene annotation information and nucleotide sequences, we found that HvWOX3A (HORVU.MOREX.r3.5HG0467090) had a G-to-A substitution at the second exon in the m-876 mutant, resulting in a change of the coding amino acid from Tryptophan to a premature stop codon at the 200th amino acid position. Remarkably, the mutation of the HvWOX3A gene leads to changes in gene expression in the m-876 mutant. Collectively, our results indicate that the loss function of the HORVU.MOREX.r3.5HG0467090 gene might be responsible for the phenotypic variation in barley mutants.