Improving Nutrient Uptake, Growth, Yield and Protein Content in Chickpea by the Co-Addition of Phosphorus Fertilizers, Organic Manures, and Bacillus sp. MN-54

Abstract

The main purpose of this study was to examine the impact of different phosphorus (P) fertilizers and organic manures alone and in combination with Bacillus sp. MN-54 on growth, yield, nutrient uptake, chlorophyll (SPAD value) and crude proteins content of chickpea. The simple manure (SM), processed manure (PM), single super phosphate (SSP), and rock phosphate (RP) were applied individually and in different combinations to the soil in pots, and the chickpea seeds treated with Bacillus sp. MN-54 were sown in the selective pots. Results showed that individual use of SM, PM, SSP, RP, and strain MN-54 significantly increased (P ≤ 0.05) the nutrient uptake, growth, yield, and protein content of chickpea as compared to control treatments. While the combined use of SM or PM, SSP or RP, and MN-54 further enhanced this effect. Among different treatments, combined use of RP, PM and MN-54 proved the most effective treatment showing increase of 37.5 and 42.6% in shoot and root lengths, 43.4 and 38.3% in fresh and dry shoot weights, 36.1 and 36.5% in fresh and dry root weights, 45.8% in no. of pods, 43.6% in nodules counts, 16.0% in 100-grain weight and 31–36% nutrient uptake over control treatments. Our findings suggest that the co-addition of organic manures and P fertilizers along with plant growth promoting bacteria (i.e., Bacillus sp. MN-54) not only increases the growth and yield but also improves nodulation, nutrient uptake, and crude proteins content in chickpea.